Two split laser beams were introduced from the bottom side of the stag
e of an optical microscope, and combined at the sample position on the
stage. The straight stripes which were produced by the interference o
f the two beams were observed in the focal plane of the objective lens
. The distance between two adjacent stripes was 630nm and gave the sca
le for two-dimensional microscopic image, and the displacement of the
stripes gave us information about the thickness and/or concentration o
f the sample. This optical scale is the measure for dimensional scalin
g of a microbody in sub-micron resolution.