INACTIVATION OF BLEOMYCIN BY AN N-ACETYLTRANSFERASE IN THE BLEOMYCIN-PRODUCING STRAIN STREPTOMYCES-VERTICILLUS

Authors
SUGIYAMA M KUMAGAI T SHIONOYA M KIMURA E DAVIES JE
Citation
M. Sugiyama et al., INACTIVATION OF BLEOMYCIN BY AN N-ACETYLTRANSFERASE IN THE BLEOMYCIN-PRODUCING STRAIN STREPTOMYCES-VERTICILLUS, FEMS microbiology letters, 121(1), 1994, pp. 81-85
Citations number
7
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
121
Issue
1
Year of publication
1994
Pages
81 - 85
Database
ISI
SICI code
0378-1097(1994)121:1<81:IOBBAN>2.0.ZU;2-J
Abstract
Bleomycin-producing Streptomyces verticillus ATCC15003 possesses a ble omycin acetyltransferase which inactivates the drug in the presence of acetyl coenzyme A. The site of acylation in enzymically prepared acet ylbleomycin A2 was determined by nuclear magnetic resonance analysis; the primary amino group of the beta-aminoalanine moiety of bleomycin w as acetylated. Acetylbleomycin A2 had no detectable antibacterial acti vity and did not induce in vitro DNA degradation.