COPPER-ZINC SUPEROXIDE-DISMUTASE FROM ASCARIS-SUUM (NEMATODA) - PURIFICATION AND CHARACTERIZATION

Authors
SANCHEZMORENO M ENTRALA E JANSSEN D OSUNA A
Citation
M. Sanchezmoreno et al., COPPER-ZINC SUPEROXIDE-DISMUTASE FROM ASCARIS-SUUM (NEMATODA) - PURIFICATION AND CHARACTERIZATION, Bioscience reports, 14(2), 1994, pp. 83-90
Citations number
13
Categorie Soggetti
Biology
Journal title
Bioscience reports → ACNP
ISSN journal
01448463
Volume
14
Issue
2
Year of publication
1994
Pages
83 - 90
Database
ISI
SICI code
0144-8463(1994)14:2<83:CSFA(->2.0.ZU;2-7
Abstract
Copper-zinc superoxide dismutase from Ascaris suum (Nematoda) was puri fied in a new, more efficient. and Easter manner. The process included differential centrifugation, fractionation with ammonium sulfate, and sodium dodecyl sulfate-polyacrylamide electrophoresis, yielding a 340 -fold purification (specific activity of 47 units/mg). Optimal storage conditions, optimal pH range, thermostability, molecular weight and u ltravioltet-visible absorption spectrum of the enzyme are described, a nd a new enzymatic model for pharmacological screening is suggested.