ADENOVIRUSES ISOLATED IN THE STOCKHOLM AREA DURING 1987-1992 - RESTRICTION-ENDONUCLEASE ANALYSIS AND MOLECULAR EPIDEMIOLOGY

Adenoviruses recovered from the northern Stockholm area during 1987-19 92 have been subjected to restriction endonuclease analysis. Adenoviru ses of all subgenera (A-F) were represented and a considerable degree of serotype variation was seen, e.g. the rarely encountered subgenus A viruses were frequently isolated in the present study. Of 16 subgenus A isolates, Ad31 predominated with 12 strains which were equally dist ributed into the DNA-variants D2 and D7. Analysis of 38 Ad3 isolates r evealed four DNA-variants: D1, D3, D10, and ''Sto1''. The ten Ad7 isol ates belonged all to the DNA-variant D5 of Ad7. Of 27 Ad1 strains, 11 belonged to D10, followed by the DNA-variants D4 and D7 with four stra ins each. Among Ad2 isolates, D2 or D2-like strains prevailed (23/28). Of six Ad5 strains, three belonged to the DNA-variant D2. The most no table subgenus D event was a nosocomial outbreak of keratokonjunctivit is due ot Ad19a. In addition, a collection of heterogenous subgenus D strains was detected, most of which untypable by RE-analysis. Among th e six Ad4 isolates of subgenus E, both genomic clusters (p and a, resp ectively) of Ad4 were recognized. Concerning the clinical important su bgenus F adenoviruses, only two strains of Ad40 were detected as compa red to 12 strains of Ad41, all of which ascribed to the DNA-variant D1 2 of Ad41.