IDENTIFICATION OF A NOVEL CELL-TYPE AND CONTEXT-SPECIFIC ENHANCER WITHIN THE NEGATIVE REGULATORY ELEMENT OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 LONG TERMINAL REPEAT

Citation
Sk. Sikder et al., IDENTIFICATION OF A NOVEL CELL-TYPE AND CONTEXT-SPECIFIC ENHANCER WITHIN THE NEGATIVE REGULATORY ELEMENT OF THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 LONG TERMINAL REPEAT, Archives of virology, 137(1-2), 1994, pp. 139-147
Citations number
32
Categorie Soggetti
Virology
Journal title
ISSN journal
03048608
Volume
137
Issue
1-2
Year of publication
1994
Pages
139 - 147
Database
ISI
SICI code
0304-8608(1994)137:1-2<139:IOANCA>2.0.ZU;2-G
Abstract
One segment of the negative regulatory region of the human immunodefic iency virus long terminal repeat (HIV-LTR), nucleotides -273 to -255, is homologous to positive response elements for interleukin-2 and gluc ocorticoids. We demonstrate that this sequence, referred to as purine- rich response element (PRRE), acts as a classic enhancer in the contex t of a heterologous promoter, but serves as a functional repressor in the intact provirus, binding (a) cellular factor(s). These data sugges t that certain DNA response elements in the HIV LTR serve as negative regulators only in the context of the intact LTR, presumably through i nteractions with other transcriptional factors, a phenomenon that migh t be exploited to interfere with viral gene transcription.