ISOLATION AND QUANTITATION OF LONG-CHAIN ACYL-COENZYME-A ESTERS IN BRAIN-TISSUE BY SOLID-PHASE EXTRACTION

Long-chain acyl-CoA's are important intermediates in fatty acid oxidat ion and phospholipid metabolism. For quantitative analysis of brain ac yl-CoA's, and to avoid decomposition due to high brain acyl-CoA hydrol ase activity, a fast and efficient analytical method was developed for isolation and determination of acyl-CoA's. The analysis includes soli d-phase extraction by an oligonucleotide purification cartridge and HP LC measurements using a synthetic internal standard. Estimates of conc entration in rat brain are oleoyl-CoA (11.0 nmol/g), palmitoyl-CoA (6. 0 nmol/g), stearoyl-CoA (4.0 nmol/g), and linoleoyl and arachidonoyl-C oA (2.0 nmol/g) for a total concentration of 23 nmol/g brain. (C) 1994 Academic Press, Inc.