IODIXANOL - A NONIONIC ISOSMOTIC CENTRIFUGATION MEDIUM FOR THE FORMATION OF SELF-GENERATED GRADIENTS

The physical and biological properties of Iodixanol, a new nonionic de nsity gradient medium, are described in this paper. It is effectively a dimer of Nycodenz and it exhibits two significant advantages over pr evious iodinated density gradient media-its aqueous solutions are iso- osmotic up to a density of 1.32 g/ml and it is capable of forming self -generating gradients in 1 to 3 h. It has a very low toxicity toward b iological material and enzyme assays can be carried out in its presenc e. (C) 1994 Academic Press, Inc.