La. Mersondavies et E. Cundliffe, ANALYSIS OF 5 TYLOSIN BIOSYNTHETIC GENES FROM THE TYIIBA REGION OF THE STREPTOMYCES-FRADIAE GENOME, Molecular microbiology, 13(2), 1994, pp. 349-355
The tyllBA region of the tylosin biosynthetic gene cluster of Streptom
yces fradiae contains at least five open reading frames (ORFs). ORF1 (
tyll) encodes a cytochrome P450 and mutations in this gene affect macr
olide ring hydroxylation. The product of ORF2 (tylB) belongs to a wide
spread family of proteins whose functions are speculative, although ty
lB mutants are defective in the biosynthesis or addition of mycaminose
during tylosin production. ORFs 3 and 4 (tylA1 and tylA2) encode delt
a TDP-glucose synthase and delta TDP-glucose dehydratase, respectively
, enzymes responsible for the first two steps common to the biosynthes
is of all three deoxyhexose sugars of tylosin via the common intermedi
ate, delta TDP-4-keto, 6-deoxyglucose. ORF5 encodes a thioesterase sim
ilar to one encoded in the erythromycin gene cluster of Saccharopolysp
ora erythraea.