Citation
Td. Visser et Jl. Oud, VOLUME MEASUREMENTS IN 3-DIMENSIONAL MICROSCOPY, Scanning, 16(4), 1994, pp. 198-200
Citations number
1
Categorie Soggetti
Microscopy
ISSN journal
01610457
Volume
16
Issue
4
Year of publication
1994
Pages
198 - 200
Database
ISI
SICI code
0161-0457(1994)16:4<198:VMI3M>2.0.ZU;2-P
Abstract
A refractive index mismatch between the oil immersion and the microsco
pic object can lead to a severe over-estimation of the object's size.
The cause of this effect is explained and a simple calibration method
to compensate for its occurrence is presented. A practical example is
discussed. The analysis applies to both conventional three-dimensional
, and confocal microscopy.