CHARACTERIZATION OF THE PROMOTER REGION OF THE GENE FOR THE RAT NEUTRAL AND BASIC-AMINO-ACID TRANSPORTER AND CHROMOSOMAL LOCALIZATION OF THE HUMAN GENE

Authors
YAN N MOSCKOVITZ R GERBER LD MATHEW S MURTY VVVS TATE SS UDENFRIEND S
Citation
N. Yan et al., CHARACTERIZATION OF THE PROMOTER REGION OF THE GENE FOR THE RAT NEUTRAL AND BASIC-AMINO-ACID TRANSPORTER AND CHROMOSOMAL LOCALIZATION OF THE HUMAN GENE, Proceedings of the National Academy of Sciences of the United Statesof America, 91(16), 1994, pp. 7548-7552
Citations number
28
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
91
Issue
16
Year of publication
1994
Pages
7548 - 7552
Database
ISI
SICI code
0027-8424(1994)91:16<7548:COTPRO>2.0.ZU;2-H
Abstract
The promoter region of the rat kidney neutral and basic amino acid tra nsporter (NBAT) gene has been isolated and sequenced. The major transc ription initiation site was mapped by primer extension. The entire pro moter region and a set of 5' deletions within it were expressed at a h igh level in LLC-PK1 cells using the luciferase indicator gene. Positi ve and negative regulatory elements in the promoter region were observ ed. A human genomic clone of the transporter was also obtained and was used to localize the NBAT gene at the p21 region of chromosome 2.