O-GLYCOSIDICALLY LINKED N-ACETYLGLUCOSAMINE-BOUND OLIGOSACCHARIDES FROM GLYCOPROTEINS OF TRYPANOSOMA-CRUZI

Citation
Jo. Previato et al., O-GLYCOSIDICALLY LINKED N-ACETYLGLUCOSAMINE-BOUND OLIGOSACCHARIDES FROM GLYCOPROTEINS OF TRYPANOSOMA-CRUZI, Biochemical journal, 301, 1994, pp. 151-159
Citations number
48
Categorie Soggetti
Biology
Journal title
ISSN journal
02646021
Volume
301
Year of publication
1994
Part
1
Pages
151 - 159
Database
ISI
SICI code
0264-6021(1994)301:<151:OLNOF>2.0.ZU;2-Z
Abstract
In this report we describe studies on the structures of the O-linked c arbohydrate units in cell-surface glycoproteins of epimastigote forms of the G-strain of Trypanosoma cruzi. Mild alkaline reductive degradat ion of the 38/43 kDa glycoproteins resulted in beta-elimination of gly cosylated threonine and/or serine residues, and the liberation of N-ac etylglucosaminitol, galactobiosyl-, galactotriosyl-, galactotetraosyl and galacto-pentaosyl-N-acetylglucosaminitol. The structures of these oligosaccharide alditols were established by n.m.r. spectroscopy and m ethylation analysis as: Galf beta 1-4(Galp beta 1-6)GlcNAc-ol Galp bet a 1-3Galp beta 1-6(Galf beta 1-4)GlcNAc-ol; [(Galp beta 1-3)(Galp beta 1-2)Galp beta 1-6](Galf beta 1-4)GlcNAc-ol; [(Galp beta 1-3)(Galp bet a 1-2)Galp beta 1-6](Galp beta 1 -2Galf beta 1-4)GlcNAc-ol.