Jo. Previato et al., O-GLYCOSIDICALLY LINKED N-ACETYLGLUCOSAMINE-BOUND OLIGOSACCHARIDES FROM GLYCOPROTEINS OF TRYPANOSOMA-CRUZI, Biochemical journal, 301, 1994, pp. 151-159
In this report we describe studies on the structures of the O-linked c
arbohydrate units in cell-surface glycoproteins of epimastigote forms
of the G-strain of Trypanosoma cruzi. Mild alkaline reductive degradat
ion of the 38/43 kDa glycoproteins resulted in beta-elimination of gly
cosylated threonine and/or serine residues, and the liberation of N-ac
etylglucosaminitol, galactobiosyl-, galactotriosyl-, galactotetraosyl
and galacto-pentaosyl-N-acetylglucosaminitol. The structures of these
oligosaccharide alditols were established by n.m.r. spectroscopy and m
ethylation analysis as: Galf beta 1-4(Galp beta 1-6)GlcNAc-ol Galp bet
a 1-3Galp beta 1-6(Galf beta 1-4)GlcNAc-ol; [(Galp beta 1-3)(Galp beta
1-2)Galp beta 1-6](Galf beta 1-4)GlcNAc-ol; [(Galp beta 1-3)(Galp bet
a 1-2)Galp beta 1-6](Galp beta 1 -2Galf beta 1-4)GlcNAc-ol.