Jn. Blankson et Ss. Morse, CD28 B7 PATHWAY COSTIMULATES THE RESPONSE OF MURINE T-CELLS TO SUPERANTIGENS AS WELL AS TO CONVENTIONAL ANTIGENS/, Cellular immunology, 157(1), 1994, pp. 306-312
While the CD28/B7 pathway has been shown to play an important costimul
atory role in the response of T cells to conventional antigens, its ro
le in superantigen-mediated T cell activation has not been as clear. I
n this report, we used CD4(+) T cells from ovalbumin-specific alpha be
ta-TCR-transgenic mice (DO 11.10) to compare the ability of this pathw
ay to costimulate the response of primary T cells to conventional anti
gens and superantigens. We show that either the addition of anti-CD28
monoclonal antibody or presentation with a B7 transfected cell line en
hances the proliferative and interleukin-2 secretion response of D011.
10 CD4(+) cells to both the conventional antigen, ovalbumin peptide (O
VA 323-339), and the superantigen SEB. This implies that CD28-mediated
costimulation plays a role in the response of murine T cells to super
antigens as well as to conventional antigens. (C) 1994 Academic Press,
Inc.