MONODISPERSE POLYMER BEADS AS PACKING MATERIAL FOR HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - EFFECT OF DIVINYLBENZENE CONTENT ON THE POROUS AND CHROMATOGRAPHIC PROPERTIES OF POLY-N-STYRENE-CO-DIVINYLBENZENE) BEADS PREPARED IN PRESENCE OF LINEAR POLYSTYRENE AS A POROGEN

The effect of concentration of divinylbenzene on pore size distributio n an sur ce areas of micropores, mesopores, and macropores in uniforml y sized porous poly (styrene- co-divinylbenzene) beads prepared in the presence of linear polystyrene as a component of the porogenic mixtur e has been studied. While the total specific surface area was clearly determined by the content of divinylbenzene, the sum of pore volumes f or mesopores and macropores as well as their size distribution does no t change within a broad range of DVB concentrations. Consequently, the size exclusion chromatography calibration curves are almost identical for all the beads prepared with different percentages of crosslinking monomer. However, the more crosslinked beads have better mechanical a nd hydrodynamic properties. (C) 1994 John Wiley & Sons, Inc.