ALTERED EXPRESSION OF SURFACE ALPHA-1,3-GLUCAN IN GENETICALLY RELATEDSTRAINS OF BLASTOMYCES-DERMATITIDIS THAT DIFFER IN VIRULENCE

Recent studies of the dimorphic fungal pathogens Histoplasma capsulatu m and Paracoccidioides brasiliensis have suggested a role in virulence for the cell surface carbohydrate alpha-(1,3)-glucan. To investigate a possible basis for alpha-(1,3)-glucan in the pathogenicity and virul ence of the dimorphic fungus Blastomyces dermatitidis, we examined thr ee genetically related strains of B. dermatitidis that differ in their virulence for mice: wild-type virulent strain ATCC 26199; mutant stra in ATCC 60915, which is 10,000 fold reduced in virulence; and mutant s train ATCC 60916, which is avirulent. Immunologic quantitation of cell wall alpha-(1,3)-glucan revealed that the mutant yeasts were almost d evoid of this sugar moiety, in contrast to the high concentration of a lpha-(1,3)-glucan on the cell wall of the wild type yeasts. These diff erences are discussed in relation to previous studies of yeast surface expression of the WI-1 antigen and recognition and binding of the rel ated strains by human monocyte-derived macrophages.