CHARACTERISTICS OF HB-VESICLES AND ENCAPSULATION PROCEDURE

Authors
TAKEOKA S SAKAI H TERASE K NISHIDE H TSUCHIDA E
Citation
S. Takeoka et al., CHARACTERISTICS OF HB-VESICLES AND ENCAPSULATION PROCEDURE, Artificial cells, blood substitutes, and immobilization biotechnology, 22(3), 1994, pp. 861-866
Citations number
8
Categorie Soggetti
Engineering, Biomedical","Materials Science, Biomaterials
Journal title
Artificial cells, blood substitutes, and immobilization biotechnology → ACNP
ISSN journal
10731199
Volume
22
Issue
3
Year of publication
1994
Pages
861 - 866
Database
ISI
SICI code
1073-1199(1994)22:3<861:COHAEP>2.0.ZU;2-Y
Abstract
The performance of Hb-vesicles depends on the weight ratio of Hb to li pid ([Hb]/[Lipid]). This value is improved by lowering the number of b ilayer membrane of the vesicle and raising the concentration of Hb in the interior of the vesicle. Maximum [Hb]/[Lipid] ratio was obtained a t ca. pH 7, that would relate to the isoelectric point (pl) of Hb at 2 5 degrees C. On the other hand, the [Hb]/[Lipid] ratio decreased with ionic strength and increased with lowering temperature. The Hb-vesicle s encapsulating 40 g/dl Hb with only one bilayer membrane were isolate d by using the difference in the density of the vesicles.