INTERLEUKIN-1-BETA INDUCES DIFFERENTIAL ADHESIVENESS ON HUMAN ENDOTHELIAL-CELL SURFACES

The adhesive strength between HL-60 and endothelial cells activated wi th IL-1 beta was investigated according to the region (area) on the en dothelial cell surface where the HL-60 cell was attached (nuclear, jun ctional, or cytoplasmic). Using a micropipette single-cell manipulatio n system, we demonstrated that the increase of adhesive force was due to the activation of the endothelial cell with IL-1 beta and a functio n of the region of the endothelial cell. The increase in adhesion stre ngth due to the increased expression of E-Selectin on the endothelial cell was studied by the addition of monoclonal antibodies against E-Se lectin, ICAM-1, VCAM, and PECAM-1. Our findings suggest that the great est adhesive strength was seen in the junctional region where the anti bodies could not block the adhesion. However, the contribution of E-Se lectin was significant in the nuclear region. (C) 1994 Academic Press, Inc.