CDNA CLONING OF HUMAN KIDNEY PYRUVATE-CARBOXYLASE

cDNA clones covering a total of 4017 bp were isolated corresponding to the full length of the message for human pyruvate carboxylase. The co ding sequence consisted of a stretch of 3534 bp flanked by a 5' untran slated sequence of 82 bp and a 3' untranslated region of 389 bp exclud ing the poly-A tail. The translated amino acid sequence of 1178 residu es contained consensus sequences for the covalent attachment for bioti n, the binding of ATP and the binding of the substrate, pyruvate. (C) 1994 Academic Press, Inc.