Ev. Bolshakova et al., CLONING AND EXPRESSION OF GENES-CODING FOR CARBOHYDRATE DEGRADING ENZYMES OF ANAEROCELLUM THERMOPHILUM IN ESCHERICHIA-COLI, Biochemical and biophysical research communications, 202(2), 1994, pp. 1076-1080
A gene library of Anaerocellum thermophilum Z-1320 was constructed in
Escherichia coli using plasmid pUC18. Clones were selected by screenin
g for hydrolysis of CMC, MU-Cel, lichenan and xylan. Recombinant clone
s expressing two beta-1,4-glucanases and two different xylanases were
obtained. Both beta-1,4-glucanases were capable of degrading Avicel. C
lones with xylanase activity also cleft PNPXyl, MU-Glu and MU-Cel. The
genes of one beta-1,4-glucanase and one xylanase are probably located
in the same region of A.thermophilum chromosome. (C) 1994 Academic Pr
ess, Inc.