CLONING AND EXPRESSION OF GENES-CODING FOR CARBOHYDRATE DEGRADING ENZYMES OF ANAEROCELLUM THERMOPHILUM IN ESCHERICHIA-COLI

Authors
BOLSHAKOVA EV PONOMARIEV AA NOVIKOV AA SVETLICHNYI VA VELIKODVORSKAYA GA
Citation
Ev. Bolshakova et al., CLONING AND EXPRESSION OF GENES-CODING FOR CARBOHYDRATE DEGRADING ENZYMES OF ANAEROCELLUM THERMOPHILUM IN ESCHERICHIA-COLI, Biochemical and biophysical research communications, 202(2), 1994, pp. 1076-1080
Citations number
9
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
202
Issue
2
Year of publication
1994
Pages
1076 - 1080
Database
ISI
SICI code
0006-291X(1994)202:2<1076:CAEOGF>2.0.ZU;2-2
Abstract
A gene library of Anaerocellum thermophilum Z-1320 was constructed in Escherichia coli using plasmid pUC18. Clones were selected by screenin g for hydrolysis of CMC, MU-Cel, lichenan and xylan. Recombinant clone s expressing two beta-1,4-glucanases and two different xylanases were obtained. Both beta-1,4-glucanases were capable of degrading Avicel. C lones with xylanase activity also cleft PNPXyl, MU-Glu and MU-Cel. The genes of one beta-1,4-glucanase and one xylanase are probably located in the same region of A.thermophilum chromosome. (C) 1994 Academic Pr ess, Inc.