Ro. Desa et al., PHYLOGENETIC ANALYSIS OF SEGMENT-10 FROM AFRICAN HORSESICKNESS VIRUS AND COGNATE GENES FROM OTHER ORBIVIRUSES, Virus research, 33(2), 1994, pp. 157-165
Utilizing the reverse transcriptase-polymerase chain reaction (RT-PCR)
procedure, we have synthesized full-length copies of segment 10 from
African horsesickness virus (AHSV) serotypes 1, 4 and 8. The genes wer
e cloned, sequenced and compared with the sequence of the cognate gene
from AHSV serotypes 3 and 9. Sequences were analyzed to assess evolut
ionary relationships among serotypes using cladistics. Based on this a
nalysis the data support a close relationship between serotypes 4 and
9 and between serotypes 1 and 8 and a closer relationship of serotype
3 to the 4 and 9 group.