We evaluated the kinetics of antibody production by turbot (Scophthalm
us maximus L.) in response to intraperitoneal (i.p.) inoculation with
Vibrio anguillarum (ATCC strain 19264) cells in the presence or absenc
e of Freund's complete adjuvant. Antibody production was monitored by
enzyme-linked immunosorbent assay (ELISA), using an anti-turbot Ig mon
oclonal antibody produced in our laboratory and three bacterial antige
n preparations (whole cells, a whole-cell extract and O-antigen). Our
results demonstrate: (a) that maximal antibody response can be achieve
d with a single inoculation in the presence of adjuvant, and is reache
d within 45 days; (b) that in the absence of adjuvant, an i.p. booster
is required to achieve a maximal response; and (c) that the whole-cel
l extract (a cytosolic fraction obtained by sonication and subsequent
ultracentrifugation at 100 000 x g for 30 min) is the best antigen for
determination by ELISA of anti-V anguillarum antibodies in turbot.