The ability of chemical irritants and several forms of physiological s
tress to induce Pecten fumatus spat to detach from settlement substrat
es was evaluated. Hypersaline baths (45 parts per thousand) and exposu
re to air (emersion) for 2 h were both found to be effective in induci
ng more than 95% of spat to detach from mesh settlement screens. Hyper
saline baths, created by the addition of an artificial sea salt to sea
water, produced greater spat detachment after 2 h than those created b
y equivalent additions of sodium chloride. The rate of detachment in h
ypersaline baths was unaffected by increasing temperature from 20-degr
ees-C to 26-degrees-C, but was depressed at 11-degrees-C. Addition of
magnesium chloride (27 g/kg) to seawater and reduction of seawater pH
to 2 were also effective in increasing spat detachment rate, but not a
s effective as hypersaline baths or air exposure. With the exception o
f spat exposed to seawater containing 115 mg/kg available chlorine, no
significant mortality and > 95% reattachment occurred within 24 h of
all detachment methods tested.