USE OF A FLAVIVIRUS RNA-DEPENDENT RNA-POLYMERASE ASSAY TO INVESTIGATETHE ANTIVIRAL ACTIVITY OF SELECTED COMPOUNDS

Authors
BARTHOLOMEUSZ A TOMLINSON E WRIGHT PJ BIRCH C LOCARNINI S WEIGOLD H MARCUCCIO S HOLAN G
Citation
A. Bartholomeusz et al., USE OF A FLAVIVIRUS RNA-DEPENDENT RNA-POLYMERASE ASSAY TO INVESTIGATETHE ANTIVIRAL ACTIVITY OF SELECTED COMPOUNDS, Antiviral research, 24(4), 1994, pp. 341-350
Citations number
34
Categorie Soggetti
Virology
Journal title
Antiviral research → ACNP
ISSN journal
01663542
Volume
24
Issue
4
Year of publication
1994
Pages
341 - 350
Database
ISI
SICI code
0166-3542(1994)24:4<341:UOAFRR>2.0.ZU;2-#
Abstract
We have developed an assay using flavivirus RNA-dependent RNA polymera se to test the inhibitory activity of potential antiviral agents. The effects of antiviral agents on RNA. synthesis were examined in this as say using extracts of Vero cells infected with dengue virus type 2 or Kunjin virus. Several different classes of known polymerase inhibitors were tested. The synthesis of double-stranded replicative form RNA wa s inhibited in a dose-dependent fashion in the presence of the polyoxo metalate HPA-23 [(NH4)(18)(NaW21Sb9O86)(17)].14 H2O and several struct urally related compounds.