THE STABILITY OF TRISUBSTITUTED INTERNUCLEOTIDE BOND IN THE PRESENCE OF THE VICINAL 2'-HYDROXYL - CHEMICAL SYNTHESIS OF URIDYL(2'-PHOSPHATE)-(3'-5')-URIDINE
R. Kierzek, THE STABILITY OF TRISUBSTITUTED INTERNUCLEOTIDE BOND IN THE PRESENCE OF THE VICINAL 2'-HYDROXYL - CHEMICAL SYNTHESIS OF URIDYL(2'-PHOSPHATE)-(3'-5')-URIDINE, Nucleosides & nucleotides, 13(8), 1994, pp. 1757-1768
The effect of eleven different phosphoryl center protecting groups on
the stability of trisubstituted internucleotide bond of the dimers (1a
-k), in the presence of the vicinal 2'-hydroxyl, was examined. It has
been found that electronic properties of the phosphoryl center protect
ing groups are essential for the reactivity of the trisubstituted inte
rnucleotide bond. Those observations were applied to the chemical synt
hesis of the uridyl(2'-phosphate)-(3'-5')-uridine, a useful model for
further pre-tRNA splicing studies.