Sp. N et al., REGENERATION OF PIGEONPEA (CAJANUS-CAJAN) FROM COTYLEDONARY NODE VIA MULTIPLE SHOOT FORMATION, Plant cell reports, 13(11), 1994, pp. 623-627
Plant regeneration, which is the major limiting factor for transformat
ion of Cajanus cajan, has been obtained via multiple shoot formation f
rom the cotyledonary node region of seedlings germinated on MS medium
containing 2 mgl(-1) 6-benzylaminopurine. A mass of multiple shoot-ini
tials formed at the axillary bud region of the cotyledonary node of th
e seedlings within two weeks. The cotyledonary node along with the mas
s of shoot-initials excised from the seedling, continued to form new s
hoot-initials on MS medium containing 6-benzylaminopurine (2 mgl(-1))
and supplemented topically with indole-3-acetic acid. The formation of
new shoot-initials was also observed from the cotyledonary nodal expl
ant, after cutting off its surface layers to completely remove the pre
-existing shoot-initials and culturing it on 6-benzylaminopurine (2 mg
l(-1)) containing medium. The shoots elongated rapidly on basal MS med
ium and rooted efficiently in MS medium supplemented with indole-3-but
yric acid (0.5 mgl(-1)). The procedure described is efficient, and hig
hly reproducible and a common response was observed for all the six Va
rieties tested.