DNA INTERACTION WITH MANGANESE IONS STUDIED BY INFRARED-SPECTROSCOPY

Citation
Sv. Kornilova et al., DNA INTERACTION WITH MANGANESE IONS STUDIED BY INFRARED-SPECTROSCOPY, Molecular biology, 27(6), 1993, pp. 791-796
Citations number
18
Categorie Soggetti
Biology
Journal title
ISSN journal
00268933
Volume
27
Issue
6
Year of publication
1993
Part
1
Pages
791 - 796
Database
ISI
SICI code
0026-8933(1993)27:6<791:DIWMIS>2.0.ZU;2-3
Abstract
DNA complexes with Mn2+ ions in films were studied by IR spectroscopy at varying relative humidity and ion concentration ([Mn2+]/[p] = 0.4-1 .0). A substantially larger number of sorbed water molecules (n = 14) was required for the DNA transition into a double-helical conformation (preferentially B form) in the presence of Mn2+ ions than without the m (n = 8). The shift of the absorption band of sorbed water to lower f requences for the DNA-Mn2+ complex may indicate tighter water binding. The Mn2+ ions were found to bind with DNA phosphates and guanine, for ming chelates of the N(7)-Mn2+-O(6) type.