SEPARATION OF 2-ARYLPROPIONIC ACIDS ON A CELLULOSE-BASED CHIRAL STATIONARY-PHASE BY RP-HPLC

The enantiomers of eight 2-arylpropionic acids, a group of chiral non steroidal antiinflammatory drugs, were resolved as their benzylamide d erivatives on a high-performance liquid chromatographic chiral station ary phase consisting of a covalently bound tris (4-methylbenzoate) cel lulose layer on silica gel. The column was used under reversed-phase c onditions using methanol as the main mobile phase component, with a pe rchlorate buffer pH 2.0. A compromise for derivatization with a water soluble carbodiimide and 1-hydroxybenzotriazole of a group of eight an alytes was obtained. The derivatives were identified by IR- and MS-spe ctroscopy.