H. Maeda et al., PRODUCTION OF L-[3-C-13] SERINE FROM [C-13] FORMALDEHYDE AND GLYCINE USING AN ENZYME-SYSTEM COMBINED WITH TETRAHYDROFOLATE REGENERATION, Journal of fermentation and bioengineering, 83(1), 1997, pp. 113-115
C-13-labelled L-serine was efficiently produced from [C-13] formaldehy
de and glycine by using partially purified L-serine hydroxymethyltrans
ferase from Methylobacterium extorquens JCM 2804, tetrahydrofolate and
a tetrahydrofolate regeneration system. The tetrahydrofolate regenera
tion system allowed efficient L-serine production and direct use of di
hydrofolate easily prepared from folic acid. A tetrahydrofolate concen
tration of more than 0.020% was necessary for efficient production. Ei
ghty seven % of the amount of [C-13] formaldehyde consumed, i.e., the
difference between the total amount of formaldehyde fed and the amount
of formaldehyde remaining in the reaction mixture, was used for the i
ncorporation into L-[C-13] serine. Seventy two mg of added glycine was
converted to L-serine when 100 mg of glycine was contained in 11.2 ml
of the final reaction mixture. The L-serine was confirmed to be L-[3-
C-13]-serine by negative fast atom bombardment mass spectrometry (FAB-
MS) and C-13-nuclear magnetic resonance spectrometry (NMR) analysis.