Sg. Altabe et al., NEW OSMOREGULATED BETA(1-3),BETA(1-6) GLUCOSYLTRANSFERASE(S) AZOSPIRILLUM-BRASILENSE, Journal of bacteriology, 176(16), 1994, pp. 4890-4898
A linear beta(1-3),beta(1-6) glucan was detected in the periplasm of A
zospirillum brasilense cells growing in a medium of low osmotic streng
th. This glucan was produced in vitro by purified bacterial inner memb
ranes with UDP-glucose as the sugar donor in the presence of Mg2+. Gro
wth in a high-osmotic-strength medium strongly reduced the amount of t
his glucan accumulated in the periplasmic space, and the inhibition wa
s associated with a reduction in the enzymatic activity of the beta(1-
3),beta(1-6) glucosyltransferase(s).