CHI-DEPENDENT FORMATION OF LINEAR PLASMID DNA IN EXONUCLEASE-DEFICIENT RECBCD(-COLI() STRAINS OF ESCHERICHIA)

Authors
ZAMAN MM BOLES TC
Citation
Mm. Zaman et Tc. Boles, CHI-DEPENDENT FORMATION OF LINEAR PLASMID DNA IN EXONUCLEASE-DEFICIENT RECBCD(-COLI() STRAINS OF ESCHERICHIA), Journal of bacteriology, 176(16), 1994, pp. 5093-5100
Citations number
49
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
176
Issue
16
Year of publication
1994
Pages
5093 - 5100
Database
ISI
SICI code
0021-9193(1994)176:16<5093:CFOLPD>2.0.ZU;2-I
Abstract
Escherichia coli strains carrying mutations in sbcB (exonuclease I) or xthA (exonuclease III) accumulate high-molecular-weight linear plasmi d concatemers when transformed with plasmids containing the chi sequen ce, 5'-GCTGGTGG-3'. Chi-dependent formation of high-molecular-weight p lasmid DNA is dependent on recA and recF functions. In addition, chi s timulation occurs only in cis. Our data are consistent with models in which RecA and RecF proteins bind to and protect the DNA ends produced by RecBCD-chi interaction.