DIRECT ELECTROCHEMICAL MEASUREMENT OF NITRIC-OXIDE PRODUCTION BY CYTOCHROME P450-CATALYZED OXIDATION OF N,N'-SUBSTITUTED HYDROXYGUANIDINES

Authors
TREVIN S ANDRE S DEVYNCK J BOUCHER JL BEDIOUI F
Citation
S. Trevin et al., DIRECT ELECTROCHEMICAL MEASUREMENT OF NITRIC-OXIDE PRODUCTION BY CYTOCHROME P450-CATALYZED OXIDATION OF N,N'-SUBSTITUTED HYDROXYGUANIDINES, Analytical communications, 34(2), 1997, pp. 69-71
Citations number
15
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical communications → ACNP
ISSN journal
13597337
Volume
34
Issue
2
Year of publication
1997
Pages
69 - 71
Database
ISI
SICI code
1359-7337(1997)34:2<69:DEMONP>2.0.ZU;2-M
Abstract
The production of nitric oxide by oxidative cleavage of N-hydroxyguani dines catalyzed by rat liver microsomes was measured directly, and for the first time by differential-pulse amperometry at a Nafion-porphyri n coated micro-sensor. The measured concentration of the nitric oxide produced (0.18-0.25 mu M) was lower than the total concentration of th e stored nitrites, The difference in the obtained data is discussed.