The expression of 65 kiloDalton heat shock protein (HSP65) immunoreact
ivity of skin biopsies from experimentally induced polymorphic light e
ruption (PLE) lesions was studied, to investigate its possible role as
a photo-induced antigen responsible for precipitating lesions. In eac
h subject the 24-h minimal erythema dose of solar simulated radiation
was determined acid an area of skin previously affected by PLE subject
ed to 70% of the minimal erythema dose in order to induce PLE lesions.
The irradiated areas were sequentially biopsied between 0 and 6 days.
ML-30, a monoclonal antibody which recognises heat shock protein 65,
was used to label the sections by means of an indirect immunoperoxidas
e technique. In PLE patients clinical inflammation was noted by 5 h po
st-irradiation, with subsequent evolution of PLE-like lesions; these w
ere still present at 6 days. Increased ML-30 antibody labelling in epi
dermal keratinocyte and endothelial cell cytoplasm was recognisable fr
om 1 h post-irradiation, and in dermal dendritic cells from 5 h sustai
ned through to 6 days. In normal subjects neither clinical nor histolo
gical features of inflammation were noted after irradiation, nor any i
ncrease in HSP65 labelling.