Ma. Domeika et al., ENZYME-IMMUNOASSAY AND DIRECT IMMUNOFLUORESCENCE FOR DETECTION OF CHLAMYDIA-TRACHOMATIS ANTIGEN IN MALE FIRST-VOID URINE, Acta dermato-venereologica, 74(4), 1994, pp. 323-326
First-void urine specimens, collected from 309 military recruits, 246
male adolescent gymnasium students and 194 patients consulting venerea
l disease clinics, were studied for the presence of Chlamydia trachoma
tis with the use of antigen detection tests two enzyme immunoassays (E
IA) and a direct immunofluorescence test (DIF; Syva MicroTrak). Urethr
al swabs were collected when discrepancies between the EIA and DIF tes
ts were detected. The patient was regarded as positive when the cultur
e result was positive or when two antigen detection tests corraborated
one another. The Syva MicroTrak EIA and DIF tests were more sensitive
than the Orion EIA, i.e. 98.5%, 99.2% and 74%, respectively. This was
true when testing both low- and high-risk groups, with a prevalence o
f chlamydial infection ranging from 0.4% to 58.6%. All three tests wer
e highly specific. The positive predictive values for the Syva MicroTr
ak EIA, the DIF and the Orion EIA were 99.2%, 100% and 100%, respectiv
ely and the negative predictive values 99.8%, 99.8% and 94.8%, respect
ively.