RECOVERY OF AMINO-ACIDS AT THE DISTAL ILEUM FOR DETERMINING APPARENT AND TRUE ILEAL AMINO-ACID DIGESTIBILITIES IN GROWING PIGS FED VARIOUS HEAT-PROCESSED FULL-FAT SOYBEAN PRODUCTS
Bj. Marty et al., RECOVERY OF AMINO-ACIDS AT THE DISTAL ILEUM FOR DETERMINING APPARENT AND TRUE ILEAL AMINO-ACID DIGESTIBILITIES IN GROWING PIGS FED VARIOUS HEAT-PROCESSED FULL-FAT SOYBEAN PRODUCTS, Journal of animal science, 72(8), 1994, pp. 2029-2037
Ten crossbred gilts fitted with simple T-cannulas at the distal ileum
were used to determine the apparent and true ileal amino acid digestib
ilities in five different soybean products: extruded, jetsploded, micr
onized, or roasted full-fat soybeans (FFSB) and soybean meal(SBM). The
gilts with an average initial body weight of 36 kg were fed the diffe
rent diets according to a replicated 5 x 5 Latin square design. Gilts
were fed twice daily at 0800 and 1830 at 2.6 times maintenance energy
requirement. All diets were cornstarch-based and formulated to contain
16% CP from one of the five soybean products. The recovery of endogen
ous lysine at the distal ileum was determined using the homoarginine t
echnique. This technique involved the guanidination of dietary lysine
to homoarginine, to allow for a differentiation between undigested die
tary lysine, represented by homoarginine, and endogenous lysine in the
digestive tract of pigs consuming diets that contain guanidinated pro
teins. Chromic oxide and dysprosium chloride were included as indigest
ible markers in the normal and;homoarginine diets, respectively. True
digestibilities were only determined with the five gilts of one Latin
square. Ileal digesta were collected for 24 h on d 8 and 10 of each 10
-d experimental period. The apparent ileal protein digestibility was h
igher in SBM than in other soybean products (P <.05). In the heat-trea
ted FFSB, the apparent protein digestibility varied between 69.0 and 8
1.6%. Recovery of endogenous lysine was affected by the diet (P <.01)
and varied between 1,329 and 2,448 mg/kg of DM intake. True lysine dig
estibility was higher in SBM(P<.05) than in roasted or jet-sploded FFS
B; extruded and micronized FFSB were intermediate. With the exclusion
of extruded FFSB, protein nitrogen flows were higher when FFSB were fe
d than when SBM was fed (P <.05). Free amino acid nitrogen flows expre
ssed as a percentage of total amino acid nitrogen flow were not influe
nced by treatment (P >.05).