D. Burgess et al., MOLECULAR-CLONING AND CHARACTERIZATION OF ADP-GLUCOSE PYROPHOSPHORYLASE CDNA CLONES ISOLATED FROM PEA COTYLEDONS, Plant molecular biology, 33(3), 1997, pp. 431-444
Three ADP-glucose pyrophosphorylase (ADPG-PPase) cDNA clones have been
isolated and characterized from a pea cotyledon cDNA library. Two of
these clones (Psagps1 and Psagps2) encode the small subunit of ADPG-PP
ase. The deduced amino acid sequences for these two clones are 95% ide
ntical. Expression of these two genes differs in that the Psagps2 gene
shows comparatively higher expression in seeds relative to its expres
sion in other tissues. Psagps2 expression also peaks midway through se
ed development at a time in which Psagps1 transcripts are still accumu
lating. The third cDNA isolated (Psagpl1) encodes the large subunit of
ADPG-PPase. It shows greater selectivity in expression than either of
the small subunit clones. It is highly expressed in sink organs (seed
, pod, and seed coat) and undetectable in leaves.