THE SIMULTANEOUS DETERMINATION OF L-697, 661, A HIV-1 SPECIFIC REVERSE-TRANSCRIPTASE INHIBITOR, AND ITS 6-HYDROXY METABOLITE IN HUMAN PLASMA VIA COLUMN-SWITCHING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

A method for the determination of L-697, 661, a human immunodeficiency virus type 1 reverse transcriptase inhibitor, and its 6-hydroxy metab olite in plasma is described. Following analyte isolation via solid ph ase extraction, samples are analyzed by HPLC using a column switching system. Column switching allows each analyte to be simultaneously anal yzed under isocratic reverse phase conditions in a run time of 21 minu tes. The assay was linear in the concentration range of 10-500 ng/mL f or each analyte when 1 mL aliquots of plasma were extracted. The assay has been fully validated and utilized for human pharmacokinetic studi es.