HIGH-DENSITY CELL-CULTURE BY MEMBRANE-BASED CELL RECYCLE

Citation
Hn. Chang et al., HIGH-DENSITY CELL-CULTURE BY MEMBRANE-BASED CELL RECYCLE, Biotechnology advances, 12(3), 1994, pp. 467-487
Citations number
76
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
ISSN journal
07349750
Volume
12
Issue
3
Year of publication
1994
Pages
467 - 487
Database
ISI
SICI code
0734-9750(1994)12:3<467:HCBMCR>2.0.ZU;2-4
Abstract
Enhancement of productivity of a bioprocess necessitates continuous op eration of bioreactors with high biomass concentrations than are possi ble in conventional batch, fed-batch or continuous modes of culture. M embrane-based cell recycle has been effectively used to maintain high cell concentrations in bioreactors. This review compares membrane-base d cell recycle operation with other such high density cell culture sys tems as immobilized cell reactors and reactors with cell recycle by ce ntrifugation or gravity sedimentation. A theoretical of production of primary and secondary metabolites in membrane-based recycle systems is presented. Operation of this type of system is discussed with example s from aerobic and anaerobic fermentations.