Methods and reagents is a unique monthly column that highlights curren
t discussions in the newsgroup bionet.molbio.methds-reagnts, available
on the Internet. This month's column discusses some problems encounte
red during the amplification of large DNA segments by the polymerase c
hain reaction (PCR), acid gives tips for doing long and accurate PCR.
For details on how to partake in the newsgroup, see the accompanying b
ox.