PURPOSE: To assess the accuracy and precision of the Barren radial vac
uum trephine as an instrument for corneal lamellar dissection. METHODS
: We describe a practical method to calibrate individual trephines pri
or to lamellar corneal procedures using the calibration microscope des
igned for diamond knives. RESULTS: We observed that one turn (360 degr
ees) of the trephine produced a mean blade displacement of 0.25mm. How
ever, individual trephines demonstrated a significant range of variabi
lity. CONCLUSION: For highly accurate trephination, it is useful to ca
librate each individual trephine using the calibration microscope.