Pd. Vanhelden et al., DETECTION BY DNA-FINGERPRINTING OF SOMATIC CHANGES DURING THE ESTABLISHMENT OF A NEW PROSTATE CELL-LINE, British Journal of Cancer, 70(2), 1994, pp. 195-198
The establishment of a new prostate cell line (BM1604) from a human pr
ostatic adenocarcinoma is reported. The line was rapidly established b
y culture of tissue on an extracellular matrix, previously laid down b
y culture of non-related cells. The method has been shown to work well
, and other prostate lines have recently been cultured in this way. Th
e cells have a doubling time of 28 h. DNA fingerprinting comparison of
the genome from the tumour, the germline and the cells shows that som
atic mutations have occurred in the tumour and that clonal selection h
as clearly occurred in establishment of the line. Many somatic mutatio
ns are apparent in the selected cells, which are now stable in culture
. This method and the cells may be a useful addition to the limited ma
terial available for the in vitro study of prostate cells.