PRESENCE OF GELATINASE A AND METALLOELASTASE TYPE PROTEASE AT THE PLASMA-MEMBRANE OF HUMAN SKIN FIBROBLASTS - INFLUENCE OF CYTOKINES AND GROWTH-FACTORS ON CELL-ASSOCIATED METALLOENDOPEPTIDASE LEVELS
Jy. Beranger et al., PRESENCE OF GELATINASE A AND METALLOELASTASE TYPE PROTEASE AT THE PLASMA-MEMBRANE OF HUMAN SKIN FIBROBLASTS - INFLUENCE OF CYTOKINES AND GROWTH-FACTORS ON CELL-ASSOCIATED METALLOENDOPEPTIDASE LEVELS, Cell biology international, 18(7), 1994, pp. 715-722
Gelatinase A and elastase type proteinase (Homsy, et al, 1988) present
at plasma membranes of human skin fibroblasts (HSF) were separated by
anion exchange chromatography on a DEAE Tris acryl M column. Elastase
type proteinase (HSFE(1)) was able to convert 72 kDa progelatinase A
to a lower 66 kDa M.W. active enzyme. Several cytokines (IL-1 beta, IL
4, IL6), interferon gamma (IFN gamma) and tumor growth factor beta (TG
F-beta) were studied for their ability to modify the levels of those p
lasma membrane associated proteinases. Among these mediators, only IL-
1 beta was found to enhance the amounts of HSF membrane-bound HSFE(1)
and Gelatinase A.