IDENTIFICATION AND CLONING OF HUMAN CHAPERONIN-10 HOMOLOG

Authors
MONZINI N LEGNAME G MARCUCCI F GROMO G MODENA D
Citation
N. Monzini et al., IDENTIFICATION AND CLONING OF HUMAN CHAPERONIN-10 HOMOLOG, Biochimica et biophysica acta, N. Gene structure and expression, 1218(3), 1994, pp. 478-480
Citations number
13
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1218
Issue
3
Year of publication
1994
Pages
478 - 480
Database
ISI
SICI code
0167-4781(1994)1218:3<478:IACOHC>2.0.ZU;2-1
Abstract
We have identified a heat-shock-inducible 10 kDa protein in the human hepatoma cell line HepG2. The total RNA extracted from the heat-shocke d cells was amplified by reverse transcription PCR (polymerase chain r eaction) using 21 5' and 18 3' oligonucleotides of rat cpn10 (chaperon in10) cDNA as primers. Sequencing of the above PCR fragment showed a v ery high homology between human, bovine and rat cpn10 cDNA. The predic ted amino acid sequence revealed a 100% identity with the bovine homol ogue.