L. Quadro et al., FREQUENT RET PROTOONCOGENE MUTATIONS IN MULTIPLE ENDOCRINE NEOPLASIA TYPE 2A, The Journal of clinical endocrinology and metabolism, 79(2), 1994, pp. 590-594
The occurrence of mutations in the RET protooncogene has been investig
ated in 12 multiple endocrine neoplasia type 2A families and 18 cases
of sporadic thyroid medullary carcinomas and pheochromocytomas. Ten of
12 families showed single base substitutions in the RET protooncogene
exons 10 and 11, coding for the extracellular domain of the protein.
Tumor tissues from 2 multiple endocrine neoplasia type 2A patients wer
e analyzed at the DNA and ribonucleic acid levels and revealed the sam
e heterozygous mutations found in the peripheral blood lymphocytes. Th
is demonstrates that both the normal and mutant alleles are expressed.
No mutations in these exons were detected in the 18 cases of sporadic
tumors investigated. These data provide further evidence that the mut
ated RET protooncogene acts in a dominant fashion and is responsible f
or the pathogenesis of this syndrome.