REGIONAL LOCALIZATION OF 725 HUMAN-CHROMOSOME-7 SPECIFIC YEAST ARTIFICIAL CHROMOSOME CLONES

Toward the construction of a complete physical map of human chromosome 7, we have localized 725 YAC clones to cytogenetically defined region s using fluorescence in situ hybridization (FISH) and by screening wit h DNA markers of known chromosomal locations. These chromosome 7-speci fic YAC clones are part of a library constructed with DNA isolated fro m monochromosomal 7 human-hamster somatic cell hybrid lines. The FISH mapping for 575 clones was accomplished by using ''ALu-PCR'' amplified YAC DNA against metaphase chromosome spreads made from a monochromoso mal 7 human-mouse somatic cell hybrid line. Hybridization- or PCR-base d screening of previously mapped DNA markers was performed for the map ping of 221 YAC clones. There was excellent correlation between the ma p locations obtained for the 71 YACs localized with both methods, All of the regionally localized YAC clones are valuable reagents for mappi ng and identification of disease genes on human chromosome 7. (C) 1994 Academic Press, Inc.