AUTOANTIBODIES AGAINST A NOVEL DNA-BINDING PROTEIN - DNA-PROTEIN INTERACTION AS A REQUISITE FOR EXPRESSION OF ANTIGENIC REACTIVITY

Authors
JUAREZ C VILA JL AGUSTI M GELPI C AMENGUAL MJ CARDONA S RODRIGUEZSANCHEZ JL
Citation
C. Juarez et al., AUTOANTIBODIES AGAINST A NOVEL DNA-BINDING PROTEIN - DNA-PROTEIN INTERACTION AS A REQUISITE FOR EXPRESSION OF ANTIGENIC REACTIVITY, Clinical immunology and immunopathology, 72(2), 1994, pp. 248-254
Citations number
25
Categorie Soggetti
Pathology,Immunology
Journal title
Clinical immunology and immunopathology → ACNP
ISSN journal
00901229
Volume
72
Issue
2
Year of publication
1994
Pages
248 - 254
Database
ISI
SICI code
0090-1229(1994)72:2<248:AAANDP>2.0.ZU;2-T
Abstract
A novel DNA-binding protein complex, the HB complex, has been characte rized by means of autoantibodies. Three proteins of 9000, 7500, and 70 00 Da constitute the HB complex. The 9000- and 7500-Da proteins are ph osphorylated. Autoantibodies recognize the 7000-Da protein when it is bound to DNA. No reactivity against any protein was observed when the complex was dissociated from DNA. The three proteins are acidic (pI 5- 6.2), and the complex was able to bind to synthetic double-stranded DN As of different composition. (C) 1994 Academic Press, Inc.