L. Breuninger et al., MONOCYTE CULTURE AND ADHERENCE MODIFIES LPS-INDUCED IL-6 PRODUCTION AND INHIBITION BY HYDROCORTISONE, Clinical immunology and immunopathology, 72(2), 1994, pp. 264-272
The effect of hydrocortisone (HC) on IL-6 production by monocytes (Mo)
that were cultured under either adherent or limited-adherent culture
conditions was determined. Although freshly isolated Mo produced more
IL-6 in response to LPS than Mo cultured 24 hr, culture had little eff
ect on inhibition of IL-6 by HC. In contrast, the conditions of Mo cul
ture had a significant impact on the kinetics of IL-6 production and i
nhibition by HC. Adherent Mo produced significantly higher levels of I
L-6 4 hr after LPS compared to limited-adherent Mo. By 12 hr of LPS st
imulation, comparable quantities of IL-6 were produced by both culture
s. HC inhibition was complete 4 hr after LPS stimulation of adherent M
o, while 12 hr was required for maximum inhibition of limited-adherent
Mo. Production of an IL-6 inhibitor was not responsible for the incre
ased inhibition by HC with adherence, because both IL-6 protein and bi
oactivity were comparably decreased. The kinetics of inhibition of TNF
alpha and IL-1 beta production by HC were similarly affected by adher
ence. Phenotypic analysis of cultured vs freshly isolated Mo showed a
decrease in Mo expressing CD14, but not CD11b, which paralleled a decr
ease in IL-6 production by the cultured Mo. However, there were no phe
notypic differences between adherent and limited-adherent Mo. The resu
lts demonstrate that the time of Mo culture, as well as adherence, may
serve to alter LPS-induced IL-6 production and its inhibition by HC.
(C) 1994 Academic Press, Inc.