STUDIES ON THE EFFECT OF AN HETEROLOGOUS FATTY-ACID-BINDING PROTEIN ON ACYL-COA OXIDASE INDUCTION IN SACCHAROMYCES-CEREVISIAE

The participation of fatty acid-binding protein (FABP) in the inductio n of peroxisomal beta-oxidation of fatty acids was investigated in viv o in an heterologous system. Bovine heart FABP was expressed in Saccha romyces cerevisiae under the control of two different promoters: a con stitutive one and an oleic acid-inducible one. Constructs were introdu ced into yeast cells on multicopy and integrating plasmids. The hetero logous FABP was present in yeast cells in two isoforms having pI value s of about 5 and was able to bind oleic acid. The heterologous FABP ha d no significant effect on acyl-CoA oxidase activity at various concen trations of the inducing agent.