CRYSTALLIZATION AND PRELIMINARY-X-RAY DIFFRACTION STUDIES OF MANDELONITRILE LYASE FROM ALMONDS

Single crystals of three different isoenzymes of (R)-(+) mandelonitril e lyase (hydroxynitrile lyase) from almonds (Prunus amygdalus) have be en obtained by hanging drop vapor diffusion using polyethylene glycol 4000 and isopropanol as co-precipitants. The crystals belong to the mo noclinic space group P2(1) with unit cell parameters a = 69.9, b = 95. 1, c = 95.6 Angstrom, and beta = 118.5 degrees. A complete set of diff raction data has been collected to 2.6 Angstrom resolution on native c rystals of isoenzyme III. (C) 1994 Wiley-Liss, Inc.