MOLECULAR-CLONING AND EXPRESSION OF 2 HORSE PANCREATIC CDNA-ENCODING COLIPASE-A AND COLIPASE-B

Pancreatic colipase plays an essential role in the intestinal fat dige stion by anchoring lipase on lipid/water interfaces in the presence of bile salts. In contrast to other species, two molecular forms of coli pase, A and B, have been found in horse. The two corresponding cDNAs w ere isolated from a horse pancreatic library and their nucleotide sequ ences were determined. Moreover, for the first time, active colipase h as been obtained after transfection of COS cells by either colipase A or B cDNA.