THE RELATIONSHIP BETWEEN THE NMDA RECEPTOR NR1 SUBUNIT MESSENGER-RNA AND [H-3] MK-801 BINDING IN THE EMBRYONIC AND EARLY POSTNATAL RAT CNS

The relationship between the NMDA receptor NR1 subunit mRNA and the bi nding of the NMDA channel ligand [H-3]MK-801 was studied during the la te embryonic and early postnatal period in various regions of the rat CNS. NR1 mRNA was detectable at embryonic day 18 in all regions analys ed and specific binding sites for [H-3]MK-801 were present in the cere bral cortex at least from the day of birth, with an unaltered affinity up to postnatal day 15. In the cortex, hippocampus and hypothalamus t here was a pronounced increase in binding between postnatal day 8 and 15, while the NR1. mRNA levels during this period were unchanged or ev en decreased. A more parallel developmental profile of mRNA content an d binding levels were seen in the medulla and spinal cord. In the cere bellum the profile was different compared to the other regions, with a clear increase in NR1 mRNA between postnatal day 8 and 15 without any change in the [H-3]MK-801 binding level. We conclude that even though the NR1 subunit alone possesses all the properties characteristic for the NMDA receptor, we cannot find a clear relationship between the le vel of its mRNA and the amount of [H-3]MK-801, binding in the developi ng rat CNS.