THE TOXIC EFFECT OF ALZHEIMER AMYLOID PROTEIN-PRECURSOR OVEREXPRESSEDIN THE NEUROBLASTOMA CELL-LINE NB-1 ON NEURITE OUTGROWTH

Authors
MARUYAMA K KUMAGAE Y SAITO Y YAMAOHARIGAYA W USAMI M ISHIURA S KAWASHIMA S OBATA K
Citation
K. Maruyama et al., THE TOXIC EFFECT OF ALZHEIMER AMYLOID PROTEIN-PRECURSOR OVEREXPRESSEDIN THE NEUROBLASTOMA CELL-LINE NB-1 ON NEURITE OUTGROWTH, Gerontology, 40, 1994, pp. 57-64
Citations number
23
Categorie Soggetti
Geiatric & Gerontology
Journal title
Gerontology → ACNP
ISSN journal
0304324X
Volume
40
Year of publication
1994
Supplement
2
Pages
57 - 64
Database
ISI
SICI code
0304-324X(1994)40:<57:TTEOAA>2.0.ZU;2-V
Abstract
The neuroblastoma cell line NB-1 is induced to start neurite outgrowth by dibutyryl cyclic AMP (Bt2cAMP). To study the function of Alzheimer amyloid protein precursor (APP), a stable cell line overexpressing AP P was established by cDNA transfection. The APP cDNA was driven by the cytomegalovirus early gene promoter. The transformant underwent degen eration as well as neurite outgrowth in the presence of Bt2cAMP, which also increased the amount of APP mRNA and protein. The expressed APP was mainly processed in the secretory pathway and few amyloidogenic fr agments were observed. Hence, not only the beta/A4 protein but also th e overexpressed APP itself might be neurotoxic.